Ricinus communis seeds harbor high oil and polyphenolics contents that hinder DNA extraction.Here, a rapid and efficient protocol for isolating total DNA from R.communis seeds was developed.The current method implies the use of repeated cycles of freeze/heat shock for the seed tissue to lyse the cells.DNA isolated with this protocol was successfull
This article describes a unique method of measuring the efficiency of gearboxes using foil strain gauges, which allows maintaining the current configuration of the gearbox within the overall assembly of the machine and its functional condition.The presented method is applicable to gearboxes located in the original equipment assembly without the nee
The aims of this analysis is to examine presuppositions on dialogue about honesty through pragmatic studies.This is a qualitative study with content analysis method because this research prioritizes the content approach of dialogue in the novel.The data source of this analysis is a dialogues that contain presuppositions and imply honesty in the nov
A flexible structure is applied to improve the lubrication performance of a misaligned journal bearing.The journal bearing is a representative sliding bearing, and there is damage Packet Caddies due to metal-to-metal contact as a result of misalignment.Since misalignment is an unavoidable phenomenon, a journal bearing with a flexible structure was